Protein - DNA Interactions

Protein - DNA Interactions

Abelson J.N., Simon M.I., Sauer R.T.
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Methods widely used in the study of DNA binding proteins are presented in this volume. These include purification and protein characterization, assays of protein-DNA binding and protein-induced bending, and biochemical and genetic methods for probing the structure, energy, and specificity of protein-DNA interactions.
Categorias:
Biology and other natural sciences - Biotechnology
Ano:
1991
Editora:
Elsevier, Academic Press
Idioma:
english
Páginas:
710
Série:
Methods in Enzymology 208
Arquivo:
PDF, 11.88 MB
IPFS:
CID , CID Blake2b
english, 1991
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    Frases chave

     
    dna
    protein
    binding
    proteins
    complex
    reaction
    nucleic
    analysis
    repressor
    specific
    interactions
    amino
    complexes
    residues
    gene
    volume
    affinity
    biol
    modification
    buffer
    containing
    linking
    polymerase
    methods
    fragment
    solution
    acids
    chemical
    footprinting
    activity
    function
    enzyme
    ligand
    rna
    studies
    experiments
    method
    concentrations
    labeled
    temperature
    edited
    rate
    mol
    cleavage
    plasmid
    salt
    sample
    equilibrium
    obtained
    effects
    promoter
    bending
    presence
    electrophoresis
    edta
    biochemistry
    column
    purification
    shown
    addition